GeneSieve™ Bioinformatics
Bar Harbor BioTechnology, Inc. is developing a data analysis suite called GeneSieve™ Bioinformatics that provides you with an intuitive interface to perform gene expression and gene profiling analysis. The approach is partially based on proprietary software which filters publicly available databases and reports useful relationships in gene expression. GeneSieve™ provides an interactive, user-friendly interface to assist you with designing experiments in the context of your biological question. Simply navigate to the GeneSieve™ website. Enter gene name, biological pathway, or gene ID number at the GeneSieve™ website and click Submit. Your query results containing links to any appropriate StellARray™ Gene Expression / CNV System plates will be e-mailed to you. GeneSieve™ Bioinformatics is available for FREE through the month of October, 2007. During this time GeneSieve™ will be running in a limited output mode, identifying only pathways that are included in existing StellARray™ plates. In this limited output mode GeneSieve™ will provide gene-based bioinformatics access to public sites such as Mouse Genome Informatics and the National Center for Biotechnology Information. GeneSieve™ will be expanded to incorporate its own interactive modules including scientific literature information, promoter analysis, and gene expression and genome position.

StellARray™ Gene Expression / CNV System
The StellARray™ Gene Expression / CNV System is a pre-manufactured standard plate loaded with oligonucleotide primers. The primers define the gene sets and are the core component of Real-Time PCR analysis. The gene sets are designed using a proprietary computer algorithm to be gene-specific and validated for optimal performance; they are applicable for gene expression as well as gene copy number (CNV) analyses. The primers for the assays are deposited robotically into each well of the plate and stabilized by proprietary methods to insure long product shelf life. The StellARrays™ are used to analyze gene expression or gene copy number by Real-Time PCR in your laboratory. Simply add your sample and SYBR Green I master mix to the StellARrays™ plates. Load the plates on your Real-Time PCR system and perform a typical run.

Global Pattern Recognition™ (GPR)
Global Pattern Recognition™ takes the Real-Time PCR data from your run and generates a ranked hit list of statistically significant changes between the test groups without reliance on the magnitude of the fold-change. This algorithm eliminates the challenges associated with the definition of a 'normalizer' gene or gene set. A normalizer (or housekeeping gene) is defined as a gene whose expression level is invariant across the biological samples being analyzed. The normalization process corrects for variances in the initial sample input quantity. This assumption (an a priori definition of a single or specific set of genes as being invariant) has been shown to be fundamentally flawed and can produce data sets that are completely arbitrary. The use of Global Pattern Recognition™ enables the experimental data to define the invariant normalizer genes, not the experimenter. Only after the genes are ranked is the magnitude of the change calculated. The magnitude is NOT the factor determining the rank. The use of any gene as a potential normalizer maximizes the use of the limited 'real-estate' on a plate. Each plate contains genes of potential value in defining the biological context without loss of wells used to contain erroneously predefined normalizers. The Global Pattern Recognition™ hit list can be analyzed once again in the GeneSieve™ system and a revised biological context is defined. The revised context may redirect the user to a new StellARray™ and thus process continues until the context stabilizes thereby redefining or validating a biological context or signature.