Bar Harbor BioTechnology, Inc. presents the GeneSieve™ Bioinformatics and Global Pattern Recognition™ Bioanalysis software packages as part of The PerfectCircle™. We developed these applications with the goal of streamlining the process of gene expression research. Your laboratory can save significant time and money in development costs that it would take to acquire a bioinformatics and bioanalysis infrastructure on its own. Use these applications in conjunction with the StellARrays™ to generate data for your gene expression research projects.

Both of these applications can be accessed through the following links:

• Access the GeneSieve™ Bioinformatics interface
• Access the Global Pattern Recognition™ interface

Application Information
Below is a brief description of the GeneSieve™ Bioinformatics and Global Pattern Recognition™ Bioanalysis software packages.

• For a more technical description, click here.

GeneSieve™
GeneSieve™ merges databases containing gene-centric biological information in a new and innovative way. With its powerful search engine capability GeneSieve™ can filter or "sieve" thousands of genes for custom population of Real-Time PCR arrays that have a unifying biological theme, such as a particular disease or pathway of interest. GeneSieve™ is incredibly easy to operate. With an intuitive user interface a subject keyword, gene ID number, or gene name is entered. All mammalian genes tagged with that search term are filtered through the GeneSieve™ modules to look for relationships. The result is a hit list of genes ranked by their relevance associated with the particular search term. GeneSieve™ then filters genes of interest using a variety of parameters such as overall score, the type of data used, literature reference, and microarray fold change. The final list of genes can then be ordered from Bar Harbor Biotechnology, Inc. as a StellARray™ populated with pre-validated Real-Time PCR oligos. Currently GeneSieve™ is available in a limited "Free-To-Use" mode. Output from GeneSieve™ will only yield StellARray™ plates. A fully customizable query version will be available soon. Register and log in to see the price per query when it is available. With the purchase of each StellARray™ pack, a credit of one GeneSieve™ query per plate will be credited to your account. Additional GeneSieve™ queries are available to be purchased as needed. Visit our for more details about pricing and availability of GeneSieve™ and StellARray™ plates. GeneSieve™ will bring a new level of search capacity to researchers with or without bioinformatics expertise.

Global Pattern Recognition™
Global Pattern Recognition™ algorithm is optimally suited to generate a ranked list of significantly changed genes within a Real-Time PCR dataset. This unique algorithm and accompanying software overcomes the problem of identifying invariant normalizers and the pitfalls of producing faulty statistics based merely on magnitude of change. Global Pattern Recognition™ provides a true statistical analysis of results based on consistency in the data, which makes Global Pattern Recognition™ optimally suited to detect small, but reproducible changes. Only after the genes are statistically ranked is the magnitude of the change calculated. Global Pattern Recognition™ is extremely simple to use and reliably tabulates statistical significance (p-value) of gene expression changes on the fly allowing you to immediately focus on the real biology. Simply log to GPR, select the StellARray™ that you ran on your Real-Time PCR instrument, upload your data and submit for analysis. An HTML or Excel formatted file will be created to download that gives a rank list of genes by p-value, fold change value, and a list of links to MGI and NCBI. With each purchase of a StellARray™ plate your account will be receive an analysis query credits sufficient to analyze each plate. Global Pattern Recognition™ will be available as an internet based software application. Through October 31, 2007 you can access it for FREE. Starting November 1, 2007 with each purchase of a StellARray™ plate your account will be receive an analysis query credit sufficient to analyze each plate. Additional Global Pattern Recognition™ analysis credits will be available to purchase.

Bar Harbor Biotechnology, Inc. is proud to present The PerfectCircle™. This suite of bioinformatics tools, Real-Time PCR arrays, and bioanalysis algorithms are a complete system for conducting gene expression research. Since all the products within The PerfectCircle™ interrelate you will no longer need to develop an ad hoc system of informatics, Real-Time PCR assays, and analysis tools. No need to purchase new equipment! No need to change your Real-Time PCR master mix vendor!